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Volume 139, Issue 6 , Pages 1674-1675 , June 2010

The murine monoclonal antibody anti-cytokeratin CAM 5.2 is specific for intracellular cytokeratin 8, not cytokeratin 18

Wen-Chang Cheng, MD
- Department of Diving Medicine, Hyperbaric Oxygen Therapy & Wound Care Center, Chung-Shan Medical University Hospital & Chung-Shan Medical University, Taichung, Taiwan, ROC
- These authors contributed equally
Jeng-Dong Hsu, DDS, MS
- Department of Pathology, Chung-Shan Medical University Hospital & Chung-Shan Medical University, Taichung, Taiwan, ROC
- School of Medicine, Chung-Shan Medical University Hospital & Chung-Shan Medical University, Taichung, Taiwan, ROC
- These authors contributed equally
Chung-Chin Yao, MD, PhD
- School of Medicine, Chung-Shan Medical University Hospital & Chung-Shan Medical University, Taichung, Taiwan, ROC
- Department of Surgery, Chung-Shan Medical University Hospital & Chung-Shan Medical University, Taichung, Taiwan, ROC
- These authors contributed equally
Chih-Ping Han, MD, PhD
- Institute of Medicinee, Department of Obstetrics and Gynecology, Clinical Trial Center, Chung-Shan Medical University Hospital & Chung-Shan Medical University, Taichung, Taiwan, ROC

References

Grotenhuis BA, Wijnhoven BP, van Marion R, van Dekken H, Hop WC, Tilanus HW, et al. The sentinel node concept in adenocarcinomas of the distal esophagus and gastroesophageal junction. J Thorac Cardiovasc Surg. 2009;138:608–612
Leica Microsystems. Novocastra lyophilized mouse monoclonal antibody cytokeratin (8/18). Product code NCL-5D3. 2008. Available at: http://www.leica-microsystems.com/products/novocastra-reagents/primary-antibodies/ http://bsd.leica-microsystems.com/pdfs/products/5d3-ce.pdf and http://www.ncl.ac.uk/business/success/spinout.php/Medical+Devices/Novocastra+Laboratories+Ltd. Accessed March 28, 2010.
Becton Dickinson. Anti-cytokeratin (CAM 5.2) reagent for in vitro diagnostic use 50 tests per vial. Catalog no. 349205, 9/97, 23-3190-01. Material safety data sheet, acc. to ISO/DIS 11014. 1997. Available at: http://www.bdbiosciences.com/external_files/is/doc/tds/Datasheets_RUO/live/web_enabled/23-1336-09-347653-347204-349205-DS-ruo.pdf. Accessed March 28, 2010.
Han CP, Hsu JD, Koo CL, Yang SF. Antibody to cytokeratin (CK8/CK18) is not derived from CAM5.2 clone, and anticytokeratin CAM5.2 (Becton Dickinson) is not synonymous with the antibody (CK8/CK18). Hum Pathol. 2010;41:616–617