The Journal of Thoracic and Cardiovascular Surgery
Volume 139, Issue 4 , Pages 1019-1025 , April 2010

Heat shock protein 27: Induction by gastroduodenal reflux in vivo and augmentation of human esophageal mucosal cell growth in vitro

Read at the Thirty-fifth Annual Meeting of The Western Thoracic Surgical Association, Banff, Alberta, Canada, June 24–27, 2009.

Received 1 July 2009 ,Revised 28 August 2009 ,Accepted 29 September 2009.

References 

  1. Brook RA, Wahlqvist P, Kleinman NL, Wallander MA, Campbell SM, Smeeding JE. Cost of gastro-oesophageal reflux disease to the employer: a perspective from the United States. Aliment Pharmacol Ther. 2007;26:889–898
  2. Zhang HY, Spechler SJ, Souza RF. Esophageal adenocarcinoma arising in Barrett esophagus. Cancer Lett. 2009;275:170–177
  3. Lee JS, Oh TY, Ahn BO, Cho H, Kim WB, Kim YB, et al. Involvement of oxidative stress in experimentally induced reflux esophagitis and Barrett's esophagus: clue for the chemoprevention of esophageal carcinoma by antioxidants. Mutat Res. 2001;480-481:189–200
  4. O'Riordan JM, Abdel-latif MM, Ravi N, McNamara D, Byrne PJ, McDonald GS, et al. Proinflammatory cytokine and nuclear factor kappa-B expression along the inflammation-metaplasia-dysplasia-adenocarcinoma sequence in the esophagus. Am J Gastroenterol. 2005;100:1257–1264
  5. Sarosiek J, McCallum RW. Mechanisms of oesophageal mucosal defence. Baillieres Best Pract Res Clin Gastroenterol. 2000;14:701–717
  6. de Vries DR, Ter Linde JJ, van Herwaarden MA, Schwartz MP, Shephard P, Geng MM, et al. GERD patients, mucosal repair associated genes are upregulated in non-inflamed oesophageal epithelium. J Cell Mol Med. 2009;13:936–947
  7. Rafiee P, Theriot ME, Nelson VM, Heidemann J, Kanaa Y, Horowitz SA, et al. Human esophageal microvascular endothelial cells respond to acidic pH stress by PI3K/AKT and p38 MAPK-regulated induction of Hsp70 and Hsp27. Am J Physiol Cell Physiol. 2006;291:C931–C945
  8. Dudeja V, Vickers SM, Saluja AK. The role of heat shock proteins in gastrointestinal diseases. Gut. 2009;58:1000–1009
  9. Soldes OS, Kuick RD, Thompson IA, Hughes SJ, Orringer MB, Iannettoni MD, et al. Differential expression of Hsp27 in normal oesophagus, Barrett's metaplasia and oesophageal adenocarcinomas. Br J Cancer. 1999;79:595–603
  10. Babu A, Meng X, Banerjee AM, Gamboni-Robertson F, Cleveland JC, Damle S, et al. Secretory phospholipase A2 is required to produce histologic changes associated with gastroduodenal reflux in a murine model. J Thorac Cardiovasc Surg. 2008;135:1220–1227
  11. Stoner GD, Kaighn ME, Reddel RR, Resau JH, Bowman D, Naito Z, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 1991;51:365–371
  12. Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P. A versatile tool for conditional gene expression and knockdown. Nat Methods. 2006;3:109–116
  13. Takubo K, Honma N, Aryal G, Sawabe M, Arai T, Tanaka Y, et al. Is there a set of histologic changes that are invariably reflux associated?. Arch Pathol Lab Med. 2005;129:159–163
  14. Fujiwara Y, Higuchi K, Tominaga K, Watanabe T, Oshitani N, Arakawa T. Functional oesophageal epithelial defense against acid. Inflammopharmacology. 2005;13:1–13
  15. Garrido C, Brunet M, Didelot C, Zermati Y, Schmitt E, Kroemer G. Heat shock proteins 27 and 70: anti-apoptotic proteins with tumorigenic properties. Cell Cycle. 2006;5:2592–2601
  16. Havasi A, Li Z, Wang Z, Martin JL, Botla V, Ruchalski K, et al. Hsp27 inhibits Bax activation and apoptosis via a phosphatidylinositol 3-kinase-dependent mechanism. J Biol Chem. 2008;283:12305–12313
  17. Wu R, Kausar H, Johnson P, Montoya-Durango DE, Merchant M, Rane MJ. Hsp27 regulates Akt activation and polymorphonuclear leukocyte apoptosis by scaffolding MK2 to Akt signal complex. J Biol Chem. 2007;282:21598–21608
  18. You W, Min X, Zhang X, Qian B, Pang S, Ding Z, et al. Cardiac-specific expression of heat shock protein 27 attenuated endotoxin-induced cardiac dysfunction and mortality in mice through a PI3K/Akt-dependent mechanism. Shock. 2009;32:108–117
  19. Beales IL, Ogunwobi O, Cameron E, El-Amin K, Mutungi G, Wilkinson M. Activation of Akt is increased in the dysplasia-carcinoma sequence in Barrett's oesophagus and contributes to increased proliferation and inhibition of apoptosis: a histopathological and functional study. BMC Cancer. 2007;7:97
  20. Oyama K, Okawa T, Nakagawa H, Takaoka M, Andl CD, Kim SH, et al. AKT induces senescence in primary esophageal epithelial cells but is permissive for differentiation as revealed in organotypic culture. Oncogene. 2007;26:2353–2364

 Supported by University of Colorado Academic Enrichment Funds.

 Disclosures: None.

PII: S0022-5223(09)01562-1

doi: 10.1016/j.jtcvs.2009.09.066

The Journal of Thoracic and Cardiovascular Surgery
Volume 139, Issue 4 , Pages 1019-1025 , April 2010

Article Tools

* Image Usage & Resolution