The Journal of Thoracic and Cardiovascular Surgery
Volume 138, Issue 3 , Pages 586-593 , September 2009

Structural and morphologic evaluation of a novel detergent–enzymatic tissue-engineered tracheal tubular matrix

  • Philipp Jungebluth

      Affiliations

    • Department of General Thoracic Surgery, Hospital Clinic, Fundació Clínic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), CIBER Enfermedades Respiratorias, Universitat de Barcelona, Barcelona, Spain
    • ,
  • Tetsuhiko Go, MD

      Affiliations

    • Department of General Thoracic Surgery, Hospital Clinic, Fundació Clínic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), CIBER Enfermedades Respiratorias, Universitat de Barcelona, Barcelona, Spain
    • ,
  • Adelaide Asnaghi

      Affiliations

    • Department of Bioengineering, Politecnico di Milano, Milano, Italy
    • ,
  • Silvia Bellini, PhD

      Affiliations

    • Department of Pharmaceutical Sciences, University of Padua, Padua, Italy
    • ,
  • Jaume Martorell, MD

      Affiliations

    • Department of Immunology, Hospital Clinic, Barcelona, Spain
    • ,
  • Chiara Calore, PhD

      Affiliations

    • Department of Pharmaceutical Sciences, University of Padua, Padua, Italy
    • ,
  • Luca Urbani

      Affiliations

    • Department of Pharmaceutical Sciences, University of Padua, Padua, Italy
    • ,
  • Helmut Ostertag, MD, PhD

      Affiliations

    • Department of Pathology, Klinikum Hannover, Hannover, Germany
    • ,
  • Sara Mantero, PhD

      Affiliations

    • Department of Bioengineering, Politecnico di Milano, Milano, Italy
    • ,
  • Maria Teresa Conconi, PhD

      Affiliations

    • Department of Pharmaceutical Sciences, University of Padua, Padua, Italy
    • ,
  • Paolo Macchiarini, MD, PhD

      Affiliations

    • Department of General Thoracic Surgery, Hospital Clinic, Fundació Clínic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), CIBER Enfermedades Respiratorias, Universitat de Barcelona, Barcelona, Spain
    • Corresponding Author InformationAddress for reprints: Paolo Macchiarini, MD, PhD, Department of General Thoracic Surgery, Hospital Clinico de Barcelona, University of Barcelona, c. Villarroel 170 E-08036 Barcelona, Spain.

Received 18 June 2008 ,Revised 2 September 2008 ,Accepted 12 September 2008.

References 

  1. Grillo HC. Tracheal replacement: a critical review. Ann Thorac Surg. 2002;73:1995–2004
  2. Gaissert HA, Grillo HC, Shadmehr MB, Wright CD, Gokhale M, Wain JC, et al. Long-term survival after resection of primary adenoid cystic and squamous cell carcinoma of the trachea and carina. Ann Thorac Surg. 2004;78:1889–1896
  3. Birchall M, Macchiarini P. Airway transplantation: a debate worth having?. Transplantation. 2008;85:1075–1080
  4. Macchiarini P. Trachea-guided generation: déjà vu all over again?. J Thorac Cardiovasc Surg. 2004;128:14–16
  5. Walles T, Giere B, Hofmann M, Schanz J, Hofmann F, Mertsching H, et al. Experimental generation of a tissue-engineered functional and vascularized trachea. J Thorac Cardiovasc Surg. 2004;128:900–906
  6. Macchiarini P, Walles T, Biancosino C, Mertsching H. First human transplantation of a bioengineered airway tissue. J Thorac Cardiovasc Surg. 2004;128:638–641
  7. Conconi MT, De Coppi P, Di Liddo R, Vigolo S, Zanon GF, Parnigotto PP, et al. Tracheal matrices, obtained by a detergent-enzymatic method, support in vitro the adhesion of chondrocytes and tracheal epithelial cells. Transpl Int. 2005;18:727–734
  8. Macchiarini P, Mazmanian GM, de Montpréville VT, Dulmet EM, Chapelier AL, Dartevelle PG. Maximal preservation time of tracheal allografts. The Paris-Sud University Lung Transplantation Group. Ann Thorac Surg. 1995;60:1597–1604
  9. Macchiarini P, Lenot B, de Montpréville VT, Dulmet E, Mazmanian GM, Fattal M, et al. Heterotopic pig model for direct revascularization and venous drainage of tracheal allografts. Paris-Sud University Lung Transplantation Group. J Thorac Cardiovasc Surg. 1994;108:1066–1075
  10. Macchiarini P, Mazmanian GM, de Montpréville VT, Dulmet E, Fattal M, Lenot B, et al. Experimental tracheal and tracheoesophageal allotransplantation. Paris-Sud University Lung Transplantation Group. J Thorac Cardiovasc Surg. 1995;110:1037–1046
  11. Solanes N, Rigol M, Khabiri E, Castella M, Ramirez J, Roque M, et al. Effects of cryopreservation on the immunogenicity of porcine arterial allografts in early stages of transplant vasculopathy. Cryobiology. 2005;51:130–141
  12. Barker E, Murison P, Macchiarini P, Jones A, Otto C, Rothkoetter HJ, et al. Early immunological changes associated with laryngeal transplantation in a major histocompatibility complex-matched pig model. Clin Exp Immunol. 2006;146:503–508
  13. Nakanishi R, Hashimoto M, Muranaka H, Yasumoto K. Effect of cryopreservation period on rat tracheal allografts. J Heart Lung Transplant. 2001;20:1010–1015
  14. Shaari CM, Farber D, Brandwein MS, Gannon P, Urken ML. Characterizing the antigenic profile of the human trachea: implications for tracheal transplantation. Head Neck. 1998;20:522–527
  15. Tojo T, Niwaya K, Sawabata N, Kushibe K, Nezu K, Taniguchi S, et al. Tracheal replacement with cryopreserved tracheal allograft: experiment in dogs. Ann Thorac Surg. 1998;66:209–213
  16. Stoelben E, Harpering H, Haberstroh J, di Filippo A, Wellens E. Heterotopic transplantation of cryopreserved tracheae in a rat model. Eur J Cardiothorac Surg. 2003;23:15–20
  17. Maeda M, Grillo HC. Effect of tension on tracheal growth after resection and anastomosis in puppies. J Thorac Cardiovasc Surg. 1973;65:658–668
  18. Marzaro M, Conconi MT, Perin L, Giuliani S, Gamba P, De Coppi P, et al. Autologous satellite cell seeding improves in vivo biocompatibility of homologous muscle acellular matrix implants. Int J Mol Med. 2002;10:177–182

 Read at the Eighty-eighth Annual Meeting of The American Association for Thoracic Surgery, San Diego, Calif, May 10–14, 2008.

 Supported by the Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, Fondo de Investigación Sanitaria (FIS) (PI050987).

PII: S0022-5223(09)00772-7

doi: 10.1016/j.jtcvs.2008.09.085

The Journal of Thoracic and Cardiovascular Surgery
Volume 138, Issue 3 , Pages 586-593 , September 2009

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