Warm-blood cardioplegic arrest induces selective mitochondrial translocation of protein kinase Cε followed by interaction with 6.1 inwardly rectifying potassium channel subunit in viable myocytes overexpressing urocortin

Chen-Scarabelli, Carol; Faggian, Giuseppe; Yuan, Zhaokan; Tessari, Maddalena; Rungatscher, Alessio; Di Rezze, Justin; Scarabelli, Gabriele M.; Abounit, Kadija; McCauley, Roy; Saravolatz, Louis; Mazzucco, Alessandro; Scarabelli, Tiziano M.

doi:10.1016/j.jtcvs.2009.03.041

« Previous Next »The Journal of Thoracic and Cardiovascular Surgery
Volume 138, Issue 5 , Pages 1213-1221, November 2009

Warm-blood cardioplegic arrest induces selective mitochondrial translocation of protein kinase Cε followed by interaction with 6.1 inwardly rectifying potassium channel subunit in viable myocytes overexpressing urocortin

Carol Chen-Scarabelli, MSc, FAHA
- VA Ann Arbor Healthcare System, University of Michigan, Mich
- Division of Cardiac Surgery, University of Verona, Verona, Italy
- Address for reprints: Carol Chen-Scarabelli, MSc, FAHA, VA Ann Arbor Healthcare System, University of Michigan, 2215 Fuller Road (111A), Ann Arbor, MI 48105.
Giuseppe Faggian, MD
- Division of Cardiac Surgery, University of Verona, Verona, Italy
Zhaokan Yuan, PhD
- Center for Heart and Vessel Preclinical Studies, St John Hospital and Medical Center, Wayne State University Medical School, Detroit, Mich
Maddalena Tessari, MSc
- Division of Cardiac Surgery, University of Verona, Verona, Italy
Alessio Rungatscher, MD
- Division of Cardiac Surgery, University of Verona, Verona, Italy
Justin Di Rezze, BSc
- Center for Heart and Vessel Preclinical Studies, St John Hospital and Medical Center, Wayne State University Medical School, Detroit, Mich
Gabriele M. Scarabelli, BSc
- Center for Heart and Vessel Preclinical Studies, St John Hospital and Medical Center, Wayne State University Medical School, Detroit, Mich
Kadija Abounit, MSc
- Center for Heart and Vessel Preclinical Studies, St John Hospital and Medical Center, Wayne State University Medical School, Detroit, Mich
Roy McCauley, PhD
- Center for Heart and Vessel Preclinical Studies, St John Hospital and Medical Center, Wayne State University Medical School, Detroit, Mich
Louis Saravolatz, MD
- Center for Heart and Vessel Preclinical Studies, St John Hospital and Medical Center, Wayne State University Medical School, Detroit, Mich
Alessandro Mazzucco, MD
- Division of Cardiac Surgery, University of Verona, Verona, Italy
Tiziano M. Scarabelli, MD, PhD, FAHA
- Center for Heart and Vessel Preclinical Studies, St John Hospital and Medical Center, Wayne State University Medical School, Detroit, Mich

Received 6 June 2008; received in revised form 23 February 2009; accepted 28 March 2009. published online 24 June 2009.

Objective

This study investigates the cardioprotective role and mechanism of action of urocortin in patients undergoing cardiac surgery, with respect to protein kinase Cε expression, activation, and relocation.

Background

Cardioplegic arrest and subsequent reperfusion inevitably expose the heart to iatrogenic ischemia/reperfusion injury. We previously reported that iatrogenic ischemia/reperfusion injury caused myocyte induction of urocortin, an endogenous cardioprotective peptide.

Methods

Two sequential biopsies were obtained from the right atrium of 25 patients undergoing coronary artery bypass grafting at the start of grafting (internal control) and 10 minutes after release of the aortic clamp.

Results

In hearts exposed to iatrogenic ischemia/reperfusion injury, induction of urocortin was documented at both the mRNA (255% of basic levels; P < .05) and the protein (4-fold increase; P < .01) levels. Iatrogenic ischemia/reperfusion injury also induced a selective increase of protein kinase Cε mRNA (225% of internal control; P < .05) and a 2-fold overexpression of total protein kinase Cε (P < .05), which paralleled a 2.9-fold increase in protein kinase Cε phosphorylation (P < .01). Mitochondrial translocation of activated protein kinase Cε was observed only in postcardioplegic samples, using both subcellular fractionation (P < .05) and immunostaining techniques (P < .05). Enhanced protein kinase Cε/mitochondria colocalization was selectively observed in viable myocytes, showing concurrently positive staining for urocortin (P < .05). Finally, co immunoprecipitation experiments documented an iatrogenic ischemia/reperfusion injury-enhanced physical interaction of phosphorylated protein kinase Cε with the 6.1 inwardly rectifying potassium channel subunit of the K_ATP channels (P < .05).

Conclusion

After iatrogenic ischemia/reperfusion injury, urocortin expression in viable cells selectively colocalized with enhanced phosphorylation and mitochondrial relocation of protein kinase Cε, suggesting a cardioprotective role for endogenous urocortin. The physical interaction of activated protein kinase Cε with 6.1 inwardly rectifying potassium channel, enhanced by cardioplegic arrest, may represent a conjectural mechanism of urocortin-mediated cardioprotection.

CTSNet classification: 18, 26

Abbreviations and Acronyms: ATP, adenosine-5′-triphosphate, CABG, coronary artery bypass graft, Hsp, heat shock protein, iIRI, iatrogenic ischemia/reperfusion injury, I/R, ischemia/reperfusion, Kir6.1, 6.1 inwardly rectifying potassium channel, Kir6.2, 6.2 inwardly rectifying potassium channel, PCR, polymerase chain reaction, PKC, protein kinase C, SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, SUR, sulfonylurea receptor, TUNEL, terminal deoxynucleotidyl transferase-mediated nick end labeling, Ucn, urocortin