The Journal of Thoracic and Cardiovascular Surgery
Volume 138, Issue 4 , Pages 985-993, October 2009

Layered implantation of myoblast sheets attenuates adverse cardiac remodeling of the infarcted heart

  • Naosumi Sekiya, MD

      Affiliations

    • Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine, Osaka, Japan
  • ,
  • Goro Matsumiya, MD, PhD

      Affiliations

    • Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine, Osaka, Japan
  • ,
  • Shigeru Miyagawa, MD, PhD

      Affiliations

    • Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine, Osaka, Japan
  • ,
  • Atsuhiro Saito, PhD

      Affiliations

    • Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine, Osaka, Japan
  • ,
  • Tatsuya Shimizu, MD, PhD

      Affiliations

    • Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan
  • ,
  • Teruo Okano, PhD

      Affiliations

    • Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan
  • ,
  • Naomasa Kawaguchi, PhD

      Affiliations

    • Department of Molecular Pathology, Osaka University Graduate School of Allied Health Science, Osaka, Japan
  • ,
  • Nariaki Matsuura, MD, PhD

      Affiliations

    • Department of Molecular Pathology, Osaka University Graduate School of Allied Health Science, Osaka, Japan
  • ,
  • Yoshiki Sawa, MD, PhD

      Affiliations

    • Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine, Osaka, Japan
    • Corresponding Author InformationAddress for reprints: Yoshiki Sawa, MD, PhD, 2-2 Yamada-oka, Suita, Osaka, 565-0871, Japan.

Received 15 May 2008; received in revised form 7 January 2009; accepted 2 February 2009.

Objective

We previously showed that autologous myoblast sheets constructed with tissue-engineering techniques improved the function of the impaired heart. In this study, we evaluated the effects of layered myoblast sheets to clarify whether increasing the number of sheets provides improvement of cardiac function.

Methods

Myoblast sheets were constructed in dishes that release confluent cells from the dish surface via temperature reduction. Sixty infarcted Lewis rats underwent implantation of myoblast sheets on the infarcted area. There were 4 groups (n = 15 in each group): S1: one layer, S3: three layers, S5: five layers, and a sham group. We examined cardiac function by echocardiography and catheterization, mRNA expression by real time reverse-transcriptase polymerase chain reaction, and histology.

Results

The ejection fraction and end-systolic pressure–volume relationship in the S5 and S3 groups were significantly improved. End-diastolic area was significantly reduced in the S5 group. The mRNAs for hepatocyte growth factor, vascular endothelial growth factor, and stromal cell–derived factor-1 were all up-regulated in dose-dependent fashion. On histologic examination, fibrosis was most decreased in S5, and vascular density was increased. Cellular hypertrophy was attenuated in both the S5 and S3 groups. Elastic fibers were massively up-regulated in the infarction and implanted sheets in the S5 and S3 groups, with expression of the elastin gene.

Conclusions

Implantation of three- and five-layered myoblast sheets yields favorable results, with better improvement of cardiac function, induction of angiogenesis, more elastic fibers, and less fibrosis. Thus, layered myoblast sheets, in optimal numbers, may attenuate adverse cardiac remodeling of the infarcted heart.

Abbreviations and Acronyms: HGF, hepatocyte growth factor, LV, left ventricular, LVEF, left ventricular ejection fraction, MI, myocardial infarction, RT-PCR, reverse-transcription polymerase chain reaction, SDF-1, stromal-derived factor 1, VEGF, vascular endothelial growth factor

CTSNet classification: 18, 29, 30

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 This work was supported by the New Energy and Industrial Technology Development Organization in Japan.

 Read at the Eighty-eighth Annual Meeting of The American Association for Thoracic Surgery, San Diego, Calif, May 10–14, 2008.

PII: S0022-5223(09)00175-5

doi:10.1016/j.jtcvs.2009.02.004

The Journal of Thoracic and Cardiovascular Surgery
Volume 138, Issue 4 , Pages 985-993, October 2009