The Journal of Thoracic and Cardiovascular Surgery
Volume 138, Issue 3 , Pages 646-653 , September 2009

Cell therapy with autologous bone marrow mononuclear stem cells is associated with superior cardiac recovery compared with use of nonmodified mesenchymal stem cells in a canine model of chronic myocardial infarction

  • Myrielle Mathieu, DVM

      Affiliations

    • Department of Physiopathology, Faculty of Medicine, UBL, Brussels, Belgium
    • Corresponding Author InformationAddress for reprints: Myrielle Mathieu, DVM, ULB–Erasme, 808 route de Lennik – CP604, 1070 Brussels, Belgium.
  • ,
  • Jozef Bartunek, MD, PhD

      Affiliations

    • Cardiovascular Center, OLV, Aalst, Belgium
    • Faculty of Biomedical Engineering, TU Eindhoven, The Netherlands
  • ,
  • Bachar El Oumeiri, MD

      Affiliations

    • Cardio-Thoracic Surgery Department, Saint-Luc University Hospital, UCL, Brussels, Belgium
  • ,
  • Karim Touihri, MD

      Affiliations

    • Department of Physiopathology, Faculty of Medicine, UBL, Brussels, Belgium
  • ,
  • Ielham Hadad, MS

      Affiliations

    • Department of Physiopathology, Faculty of Medicine, UBL, Brussels, Belgium
  • ,
  • Philippe Thoma, MD

      Affiliations

    • Department of Radiology and Medical Imaging, Faculty of Medicine, UBL, Brussels, Belgium
  • ,
  • Thierry Metens, MS, PhD

      Affiliations

    • Department of Radiology and Medical Imaging, Faculty of Medicine, UBL, Brussels, Belgium
  • ,
  • Agnes Mendes da Costa, DVM

      Affiliations

    • Department of Physiopathology, Faculty of Medicine, UBL, Brussels, Belgium
  • ,
  • Maryam Mahmoudabady, MD

      Affiliations

    • Department of Physiopathology, Faculty of Medicine, UBL, Brussels, Belgium
  • ,
  • Dominique Egrise, MS, PhD

      Affiliations

    • Department of Radio-Isotope Imaging, Faculty of Medicine, UBL, Brussels, Belgium
  • ,
  • Didier Blocklet, MD

      Affiliations

    • Department of Radio-Isotope Imaging, Faculty of Medicine, UBL, Brussels, Belgium
  • ,
  • Naïma Mazouz, MS, PhD

      Affiliations

    • Cardio3BioSciences, Braine L'Alleud, Belgium
  • ,
  • Robert Naeije, MD, PhD

      Affiliations

    • Department of Physiopathology, Faculty of Medicine, UBL, Brussels, Belgium
  • ,
  • Guy Heyndrickx, MD, PhD

      Affiliations

    • Cardiovascular Center, OLV, Aalst, Belgium
    • Department of Physiology, Saint-Luc University Hospital, UCL, Brussels, Belgium
  • ,
  • Kathleen McEntee, DVM, PhD

      Affiliations

    • Department of Physiopathology, Faculty of Medicine, UBL, Brussels, Belgium

Received 27 August 2008 ,Revised 25 November 2008 ,Accepted 25 December 2008.

References 

  1. Orlic D, Kajstura J, Chimenti S, Jakoniuk I, Anderson SM, Li B, et al. Bone-marrow cells regenerate infarcted myocardium. Nature. 2001;410:701–705
  2. Assmus B, Schächinger V, Teupe C, Britten M, Lehmann R, Döbert N, et al. Transplantation of Progenitor Cells and Regeneration Enhancement in Acute Myocardial Infarction (TOPCARE-AMI). Circulation. 2002;106:3009–3017
  3. Strauer BE, Brehm M, Zeus T, Köstering M, Hernandez A, Sorg RV, et al. Repair of infarcted myocardium by autologous intracoronary mononuclear bone-marrow cell transplantation in humans. Circulation. 2002;106:1913–1918
  4. Wollert KC, Meyer GP, Lotz J, Ringes-Lichtenberg S, Lippolt P, Breidenbach C, et al. Intracoronary autologous bone-marrow cell transfer after myocardial infarction: the BOOST randomised controlled clinical trial. Lancet. 2004;364:141–148
  5. Janssens S, Dubois C, Bogaert J, Theunissen K, Deroose C, Desmet W, et al. Autologous bone marrow-derived stem-cell transfer in patients with ST-segment elevation myocardial infarction: double-blind, randomised controlled trial. Lancet. 2006;367:113–121
  6. Schächinger V, Erbs S, Elsässer A, Haberbosch W, Hambrecht R, Hölschermann H, et al. Intracoronary bone marrow-derived progenitor cells in acute myocardial infarction. N Engl J Med. 2006;355:1210–1221
  7. Lunde K, Solheim S, Aakhus S, Arnesen H, Abdelnoor M, Egeland T, et al. Intracoronary injection of mononuclear bone-marrow cells in acute myocardial infarction. N Engl J Med. 2006;355:1199–1209
  8. Silva GV, Litovsky S, Assad JA, Sousa AL, Martin BJ, Vela D, et al. Mesenchymal stem cells differentiate into an endothelial phenotype, enhance vascular density, and improve heart function in a canine chronic ischemia model. Circulation. 2005;111:150–156
  9. Toma C, Pittenger MF, Cahill KS, Byrne BJ, Kessler PD. Human mesenchymal stem cells differentiate to a cardiomyocyte phenotype in the adult murine heart. Circulation. 2002;105:93–98
  10. Dai W, Hale SL, Martin BJ, Kuang JQ, Dow JS, Wold LE, et al. Allogeneic mesenchymal stem cell transplantation in postinfarcted rat myocardium: short- and long-term effects. Circulation. 2005;112:214–223
  11. Jiang Y, Jahagirdar BN, Reinhardt RL, Schwartz RE, Keene CD, Ortiz-Gonzalez XR, et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature. 2002;418:41–49
  12. Di Nicola M, Carlo-Stella C, Magni M, Milanesi M, Longoni PD, Matteucci P, et al. Human bone-marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli. Blood. 2002;99:3838–3843
  13. Bartunek J, Croissant JD, Wijns W, Gofflot S, de Lavareille A, Vanderheyden M, et al. Pretreatment of adult bone-marrow mesenchymal stem cells with cardiomyogenic growth factors and repair of the chronically infarcted myocardium. Am J Physiol Heart Circ Physiol. 2007;292:H1095–H1104
  14. Sahn DJ, DeMaria A, Kisslo J, Weyman A. Recommendations regarding quantitation in M-mode echocardiography: results of a survey of echocardiographic measurements. Circulation. 1978;58:1072–1083
  15. Schiller NB, Shah PM, Crawford M, De Maria A, Devereux R, Feigenbaum H, et al. Recommendations for quantitation of the left ventricle by two-dimensional echocardiography. American Society of Echocardiography Committee on Standards, Subcommittee on Quantitation of Two-Dimensional Echocardiograms. J Am Soc Echocardiogr. 1989;2:358–367
  16. Baan J, van der Velde ET, de Bruin HG, Smeenk GJ, Koops J, van Dijk AD, et al. Continuous measurement of left ventricular volume in animals and humans by conductance catheter. Circulation. 1984;70:812–823
  17. Winer J, Jung CK, Shackel I, Williams PL. Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro. Anal Biochem. 1999;270:41–49
  18. Rafii S, Lyden D. Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration. Nat Med. 2003;9:702–712
  19. Murry CE, Soonpaa MH, Reinecke H, Nakajima H, Nakajima HO, Rubart M, et al. Haematopoietic stem cells do not transdifferentiate into cardiac myocytes in myocardial infarcts. Nature. 2004;428:664–668
  20. Vanderheyden M, Heyndrickx G, Wijns W, Bartunek J. Framework towards optimisation of bone-marrow stem cells therapy for cardiac repair. Eurointervention. 2007;2:B95–B98
  21. Stamm C, Westphal B, Kleine HD, Petzsch M, Kittner C, Klinge H, et al. Autologous bone-marrow stem-cell transplantation for myocardial regeneration. Lancet. 2003;361:45–46
  22. Perin EC, Dohmann HF, Borojevic R, Silva SA, Sousa AL, Mesquita CT, et al. Transendocardial, autologous bone marrow cell transplantation for severe, chronic ischemic heart failure. Circulation. 2003;107:2294–2302
  23. Strauer BE, Brehm M, Zeus T, Bartsch T, Schannwell C, Antke C, et al. Regeneration of human infarcted heart muscle by intracoronary autologous bone-marrow cell transplantation in chronic coronary artery disease: the IACT Study. J Am Coll Cardiol. 2005;46:1651–1658
  24. Shake JG, Gruber PJ, Baumgartner WA, Senechal G, Meyers J, Redmond JM, et al. Mesenchymal stem cell implantation in a swine myocardial infarct model: engraftment and functional effects. Ann Thorac Surg. 2002;73:1919–1925
  25. Amado LC, Saliaris AP, Schuleri KH, St John M, Xie JS, Cattaneo S, et al. Cardiac repair with intramyocardial injection of allogeneic mesenchymal stem cells after myocardial infarction. Proc Natl Acad Sci U S A. 2005;102:11474–11479
  26. Moelker AD, Baks T, van den Bos EJ, van Geuns RJ, de Feyter PJ, Duncker DJ, et al. Reduction in infarct size, but no functional improvement after bone marrow cell administration in a porcine model of reperfused myocardial infarction. Eur Heart J. 2006;27:3057–3064
  27. Kawamoto A, Iwasaki H, Kusano K, Murayama T, Oyamada A, Silver M, et al. CD34-positive cells exhibit increased potency and safety for therapeutic neovascularization after myocardial infarction compared with total mononuclear cells. Circulation. 2006;114:2163–2169
  28. Grunewald M, Avraham I, Dor Y, Bachar-Lustig E, Itin A, Jung S, et al. VEGF-induced adult neovascularization: recruitment, retention, and role of accessory cells. Cell. 2006;124:175–189
  29. Urbich C, Dimmeler S. Endothelial progenitor cells functional characterization. Trends Cardiovasc Med. 2004;14:318–322
  30. Tomita S, Mickle DA, Weisel RD, Jia ZQ, Tumiati LC, Allidina Y, et al. Improved heart function with myogenesis and angiogenesis after autologous porcine bone marrow stromal cell transplantation. J Thorac Cardiovasc Surg. 2002;123:1132–1140

 This work was supported by the Foundation for Cardiac Surgery, Brussels, Belgium.

PII: S0022-5223(09)00015-4

doi: 10.1016/j.jtcvs.2008.12.031

The Journal of Thoracic and Cardiovascular Surgery
Volume 138, Issue 3 , Pages 646-653 , September 2009