The Journal of Thoracic and Cardiovascular Surgery
Volume 135, Issue 1 , Pages 8-18.e2 , January 2008

Spatiotemporal patterns of smooth muscle cell changes in ascending aortic dilatation with bicuspid and tricuspid aortic valve stenosis: Focus on cell–matrix signaling

Read at the Eighty-seventh Annual Meeting of The American Association for Thoracic Surgery, Washington, DC, May 5-9, 2007.

  • Alessandro Della Corte, MD

      Affiliations

    • Department of Cardiothoracic and Respiratory Sciences, Second University of Naples, V. Monaldi Hospital, Naples, Italy
    • PhD program, “Medical and Surgical Physiopathology of the Cardio-Respiratory System and Associated Biotechnologies,” Second University of Naples, Italy.
    • Corresponding Author InformationAddress for reprints: Alessandro Della Corte, MD, Via P. Neruda, 6, 81031 Aversa CE, Italy.
    • ,
  • Cesare Quarto, MD

      Affiliations

    • Department of Cardiothoracic and Respiratory Sciences, Second University of Naples, V. Monaldi Hospital, Naples, Italy
    • PhD program, “Medical and Surgical Physiopathology of the Cardio-Respiratory System and Associated Biotechnologies,” Second University of Naples, Italy.
    • ,
  • Ciro Bancone, MD

      Affiliations

    • Department of Cardiothoracic and Respiratory Sciences, Second University of Naples, V. Monaldi Hospital, Naples, Italy
    • ,
  • Clotilde Castaldo, MD

      Affiliations

    • Department of Biomorphological and Functional Sciences, “Federico II” University, Secondo Policlinico, Naples, Italy.
    • ,
  • Franca Di Meglio, MD

      Affiliations

    • Department of Biomorphological and Functional Sciences, “Federico II” University, Secondo Policlinico, Naples, Italy.
    • ,
  • Daria Nurzynska, MD

      Affiliations

    • Department of Biomorphological and Functional Sciences, “Federico II” University, Secondo Policlinico, Naples, Italy.
    • ,
  • Luca S. De Santo, MD

      Affiliations

    • Department of Cardiothoracic and Respiratory Sciences, Second University of Naples, V. Monaldi Hospital, Naples, Italy
    • ,
  • Marisa De Feo, MD, PhD

      Affiliations

    • Department of Cardiothoracic and Respiratory Sciences, Second University of Naples, V. Monaldi Hospital, Naples, Italy
    • ,
  • Michelangelo Scardone, MD

      Affiliations

    • Department of Cardiothoracic and Respiratory Sciences, Second University of Naples, V. Monaldi Hospital, Naples, Italy
    • ,
  • Stefania Montagnani, MD

      Affiliations

    • Department of Biomorphological and Functional Sciences, “Federico II” University, Secondo Policlinico, Naples, Italy.
    • ,
  • Maurizio Cotrufo, MD

      Affiliations

    • Department of Cardiothoracic and Respiratory Sciences, Second University of Naples, V. Monaldi Hospital, Naples, Italy

Received 1 May 2007 ,Revised 23 August 2007 ,Accepted 20 September 2007.

References 

  1. Lesauskaite V, Tanganelli P, Sassi C, Neri E, Diciolla F, Ivanoviene L, et al. Smooth muscle cells of the media in the dilatative pathology of ascending thoracic aorta: morphology, immunoreactivity for osteopontin, matrix metalloproteinases, and their inhibitors. Hum Pathol. 2001;32:1003–1011
  2. Bonderman D, Gharehbaghi-Schnell E, Wollenek G, Maurer G, Baumgartner H, Lang IM. Mechanisms underlying aortic dilatation in congenital aortic valve malformation. Circulation. 1999;99:2138–2143
  3. Ikonomidis JS, Jones JA, Barbour JR, Stroud RE, Clark LL, Kaplan BS, et al. Expression of matrix metalloproteinases and endogenous inhibitors within ascending aortic aneurysms of patients with bicuspid or tricuspid aortic valves. J Thorac Cardiovasc Surg. 2007;133:1028–1036
  4. Cotrufo M, Della Corte A, De Santo LS, Quarto C, De Feo M, Romano G, et al. Different patterns of extracellular matrix protein expression in the convexity and the concavity of the dilated aorta with bicuspid aortic valve: preliminary results. J Thorac Cardiovasc Surg. 2005;130:504–511
  5. Della Corte A, Bancone C, Quarto C, Dialetto G, Covino FE, Scardone M, et al. Predictors of ascending aortic dilatation with bicuspid aortic valve: a wide spectrum of disease expression. Eur J Cardiothorac Surg. 2007;31:397–405
  6. Davies RR, Kaple RK, Mandapati D, Gallo A, Botta DM, Elefteriades JA, et al. Natural history of ascending aortic aneurysms in the setting of an unreplaced bicuspid aortic valve. Ann Thorac Surg. 2007;83:1338–1344
  7. Zhang Z, Vuori K, Reed JC, Ruoslahti E. The alpha 5 beta 1 integrin supports survival of cells on fibronectin and up-regulates Bcl-2 expression. Proc Natl Acad Sci U S A. 1995;92:6161–6165
  8. Thyberg J, Hultgardh-Nilsson A. Fibronectin and the basement membrane components laminin and collagen type IV influence the phenotypic properties of subcultured rat aortic smooth muscle cells differently. Cell Tissue Res. 1994;276:263–271
  9. Michel JB. Anoikis in the cardiovascular system: known and unknown extracellular mediators. Arterioscler Thromb Vasc Biol. 2003;23:2146–2154
  10. Martin SS, Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004;1692:145–157
  11. Chen CS, Mrksich M, Huang S, Whitesides GM, Ingber DE. Geometric control of cell life and death. Science. 1997;276:1425–1428
  12. Scholzen T, Gerdes J. The Ki-67 protein: from the known and the unknown. J Cell Physiol. 2000;182:311–322
  13. Puthalakath H, Villunger A, O’Reilly LA, Beaumont JG, Coultas L, Cheney RE, et al. Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis. Science. 2001;293:1829–1832
  14. Frisch SM, Screaton RA. Anoikis mechanisms. Curr Opin Cell Biol. 2001;13:555–562
  15. Borger MA, Preston M, Ivanov J, Fedak PW, Davierwala P, Armstrong S, et al. Should the ascending aorta be replaced more frequently in patients with bicuspid aortic valve disease?. J Thorac Cardiovasc Surg. 2004;128:677–683
  16. McKellar SH, Tester DJ, Yagubyan M, Majumdar R, Ackerman MJ, Sundt TM. Novel NOTCH1 mutations in patients with bicuspid aortic valve disease and thoracic aortic aneurysms. J Thorac Cardiovasc Surg. 2007;134:290–296
  17. Tang PC, Coady MA, Lovoulos C, Dardik A, Aslan M, Elefteriades JA, et al. Hyperplastic cellular remodeling of the media in ascending thoracic aortic aneurysms. Circulation. 2005;112:1098–1105
  18. Kirsch EW, Radu NC, Gervais M, Allaire E, Loisance DY. Heterogeneity in the remodeling of aneurysms of the ascending aorta with tricuspid aortic valves. J Thorac Cardiovasc Surg. 2006;132:1010–1016
  19. Ihling C, Szombathy T, Nampoothiri K, Haendeler J, Beyersdorf F, Uhl M, et al. Cystic medial degeneration of the aorta is associated with p53 accumulation, Bax upregulation, apoptotic cell death, and cell proliferation. Heart. 1999;82:286–293
  20. Jones PL, Jones FS, Zhou B, Rabinovitch M. Induction of vascular smooth muscle cell tenascin-C gene expression by denatured type I collagen is dependent upon a beta3 integrin-mediated mitogen-activated protein kinase pathway and a 122-base pair promoter element. J Cell Sci. 1999;112:435–445
  21. Wallner K, Li C, Shah PK, Wu KJ, Schwartz SM, Sharifi BG. EGF-like domain of tenascin-C is proapoptotic for cultured smooth muscle cells. Arterioscler Thromb Vasc Biol. 2004;24:1416–1421
  22. Flusberg DA, Numaguchi Y, Ingber DE. Cooperative control of Akt phosphorylation, bcl-2 expression, and apoptosis by cytoskeletal microfilaments and microtubules in capillary endothelial cells. Mol Biol Cell. 2001;12:3087–3094
  23. Boudreau NJ, Jones PL. Extracellular matrix and integrin signalling: the shape of things to come. Biochem J. 1999;339:481–488
  24. Birney YA, Sweeney CH, Cappadona CR, Sitzmann JV, Cummins PM, Redmond EM, et al. Pulse pressure-induced transmural fluid flux increases bovine aortic smooth muscle cell apoptosis in a mitogen activated protein kinase dependent manner. J Vasc Res. 2004;41:364–374
  25. Donal E, Novaro GM, Deserranno D, Popovic ZB, Greenberg NL, Richards KE, et al. Planimetric assessment of anatomic valve area overestimates effective orifice area in bicuspid aortic stenosis. Am J Soc Echocardiogr. 2005;18:1392–1398
  26. Robicsek F, Thubrikar MJ, Cook JW, Fowler B. The congenitally bicuspid aortic valve: How does it function? (Why does it fail?). Ann Thorac Surg. 2004;77:177–185
  27. Williams B. Mechanical influences on vascular smooth muscle cell function. J Hypertens. 1998;16:1921–1929
  28. Grote K, Flach I, Luchtefeld M, Akin E, Holland SM, Drexler H, et al. Mechanical stretch enhances mRNA expression and proenzyme release of matrix metalloproteinase-2 (MMP-2) via NAD(P)H oxidase-derived reactive oxygen species. Circ Res. 2003;92:80–86
  29. Wernig F, Mayr M, Xu Q. Mechanical stretch-induced apoptosis in smooth muscle cells is mediated by β-1-integrin signalling pathways. Hypertension. 2003;41:903–911
  30. Chambers RC, Leoni P, Kaminski N, Laurent GJ, Heller RA. Global expression profiling of fibroblast responses to transforming growth factor-beta1 reveals the induction of inhibitor of differentiation-1 and provides evidence of smooth muscle cell phenotypic switching. Am J Pathol. 2003;162:533–546

 This study was supported by a grant (P.R.I.N. co-funding 2006; prot. 2006063487) from the Italian Ministry for University and Research.

PII: S0022-5223(07)01545-0

doi: 10.1016/j.jtcvs.2007.09.009

The Journal of Thoracic and Cardiovascular Surgery
Volume 135, Issue 1 , Pages 8-18.e2 , January 2008

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