The Journal of Thoracic and Cardiovascular Surgery
Volume 133, Issue 5 , Pages 1147-1153 , May 2007

Cellular coating of the left ventricular assist device textured polyurethane membrane reduces adhesion of Staphylococcus aureus

  • Tomohiro Asai, MD

      Affiliations

    • Department of Surgery, New York Presbyterian Hospital, College of Physicians & Surgeons, Columbia University, New York, NY
    • ,
  • Mei-Ho Lee

      Affiliations

    • Department of Medicine, New York Presbyterian Hospital, College of Physicians & Surgeons, Columbia University, New York, NY
    • ,
  • Carlos Arrecubieta, PhD

      Affiliations

    • Department of Medicine, New York Presbyterian Hospital, College of Physicians & Surgeons, Columbia University, New York, NY
    • ,
  • Manuel Prinz von Bayern

      Affiliations

    • Department of Medicine, New York Presbyterian Hospital, College of Physicians & Surgeons, Columbia University, New York, NY
    • ,
  • Christian A. Cespedes

      Affiliations

    • Department of Medicine, New York Presbyterian Hospital, College of Physicians & Surgeons, Columbia University, New York, NY
    • ,
  • Helen M. Baron, MD

      Affiliations

    • Department of Medicine, New York Presbyterian Hospital, College of Physicians & Surgeons, Columbia University, New York, NY
    • ,
  • Martin Cadeiras, MD

      Affiliations

    • Department of Medicine, New York Presbyterian Hospital, College of Physicians & Surgeons, Columbia University, New York, NY
    • ,
  • Taichi Sakaguchi, MD

      Affiliations

    • Department of Surgery, New York Presbyterian Hospital, College of Physicians & Surgeons, Columbia University, New York, NY
    • ,
  • Charles C. Marboe, MD

      Affiliations

    • Department of Pathology, New York Presbyterian Hospital, College of Physicians & Surgeons, Columbia University, New York, NY.
    • ,
  • Yoshifumi Naka, MD, PhD

      Affiliations

    • Department of Surgery, New York Presbyterian Hospital, College of Physicians & Surgeons, Columbia University, New York, NY
    • ,
  • Mario C. Deng, MD

      Affiliations

    • Department of Medicine, New York Presbyterian Hospital, College of Physicians & Surgeons, Columbia University, New York, NY
    • Corresponding Author InformationAddress for reprints: Mario C. Deng, MD, FACC, FESC, Director of Cardiac Transplantation Research, Department of Medicine, Columbia University College of Physicians & Surgeons, New York, NY 10032.
    • ,
  • Franklin D. Lowy, MD

      Affiliations

    • Department of Medicine, New York Presbyterian Hospital, College of Physicians & Surgeons, Columbia University, New York, NY
    • Department of Pathology, New York Presbyterian Hospital, College of Physicians & Surgeons, Columbia University, New York, NY.

Received 14 June 2006 ,Revised 7 October 2006 ,Accepted 25 October 2006.

  • Image Result

    Bacterial density on surface of polyurethane membrane 3 days after inoculation. A, Animals were inoculated with different concentrations of S aureus (106 and 107 colony-forming units [CFU]/mouse). B,

    Bacterial density on surface of polyurethane membrane 3 days after inoculation. A, Animals were inoculated with different concentrations of S aureus (106 and 107 colony-forming units [CFU]/mouse). B, Animals were infected days 1 and 14 after polyurethane patch implantation. pod, Postoperative day.

  • Image Result
    Bacterial density in organs 3 days after infection with S aureus. A–C, Animals were inoculated with different concentrations of S aureus (106 and 107 colony-forming units [CFU]/mouse). D–F, Animals we

    Bacterial density in organs 3 days after infection with S aureus. A–C, Animals were inoculated with different concentrations of S aureus (106 and 107 colony-forming units [CFU]/mouse). D–F, Animals were infected days 1 and 14 after polyurethane patch implantation. pod, Postoperative day.

  • Image Result
    A, Representative cross-section of aorta (Ao) 1 day after polyurethane patch (P) implantation, covered with thin fibrin (f) layer (hematoxylin-eosin staining, original magnification ×50). B, Represent

    A, Representative cross-section of aorta (Ao) 1 day after polyurethane patch (P) implantation, covered with thin fibrin (f) layer (hematoxylin-eosin staining, original magnification ×50). B, Representative cross-section of aorta (Ao) 4 days after polyurethane patch (P) implantation, covered with thick fibrin (f) layer. Animal was infected 1 day after implantation (hematoxylin-eosin staining, original magnification ×50) PMN, Polymorphonuclear cells. C, Cross-section obtained from same infected animal was Gram stained (107 colony-forming units, original magnification ×1000). Arrows indicate gram-positive cocci. P, Polyurethane patch.

  • Image Result
    A, Hematoxylin-eosin staining of representative section of implanted polyurethane membrane (P) 1 day after implantation in aorta (Ao). Blood-contacting surface of polyurethane membrane is covered with

    A, Hematoxylin-eosin staining of representative section of implanted polyurethane membrane (P) 1 day after implantation in aorta (Ao). Blood-contacting surface of polyurethane membrane is covered with thin layer of fibrin (f), and polymorphonuclear cells (PMN) are embedded with fibrin. Inserts show polymorphonuclear leukocytes. B, Hematoxylin-eosin staining of representative section of implanted polyurethane membrane (P) 14 days after implantation in aorta (Ao). Blood-contacting surface of polyurethane membrane is covered with cells. C, CD31 staining of representative section of implanted polyurethane membrane (P) 14 days after implantation in aorta (Ao) reveals cells on luminal surface to be CD31+ (arrows). This suggests that after 14 days polyurethane membrane has been covered with protective cell layer. These findings were consistent for all animals examined (n = 4 in each group; original magnification ×200). Insert shows 1000× magnification of CD31+ cells.

  • Image Result
    In vitro assay. Polyurethane membranes were fixed to 96-well tissue culture dishes and coated with fibrinogen. Half of membranes were subsequently coated with human endothelial cells and grown to conf

    In vitro assay. Polyurethane membranes were fixed to 96-well tissue culture dishes and coated with fibrinogen. Half of membranes were subsequently coated with human endothelial cells and grown to confluence. Wb strain S aureus was added to wells, and adhesion assay was performed. CFU, Colony-forming units.

 Supported by the National Heart, Lung, and Blood institute (NHLBI) Specialized Center for Clinically Oriented Research (SCCOR) grant HL 077096-01, named “The Biology of Human Long-Term Mechanical Circulatory Support,” and by The Health Research Board program grant PRO 09/2002.

PII: S0022-5223(07)00130-4

doi: 10.1016/j.jtcvs.2006.10.084

The Journal of Thoracic and Cardiovascular Surgery
Volume 133, Issue 5 , Pages 1147-1153 , May 2007

Article Tools

* Image Usage & Resolution