The Journal of Thoracic and Cardiovascular Surgery
Volume 133, Issue 1 , Pages 37-43 , January 2007

Roles of cyclooxygenase-2 and phosphorylated Akt (Thr308) in cardiac hypertrophy regression mediated by left-ventricular unloading

  • Jeremias Wohlschlaeger, MD

      Affiliations

    • Department of Pathology and Neuropathology, University Hospital Essen, University of Duisburg-Essen, Germany
    • J. W. and K. J. S. contributed equally to the study.
    • ,
  • Klaus Jürgen Schmitz, MD

      Affiliations

    • Department of Pathology and Neuropathology, University Hospital Essen, University of Duisburg-Essen, Germany
    • J. W. and K. J. S. contributed equally to the study.
    • ,
  • Jenci Palatty

      Affiliations

    • Department of Pathology and Neuropathology, University Hospital Essen, University of Duisburg-Essen, Germany
    • ,
  • Atsushi Takeda, MD

      Affiliations

    • Faculty of Health Science, School of Physical Therapy, Gumma Paz College, Gumma, Japan
    • ,
  • Nobuakira Takeda, MD

      Affiliations

    • Department of Internal Medicine, Jikei University, Tokyo, Japan
    • ,
  • Christian Vahlhaus, MD

      Affiliations

    • Department of Cardiology and Angiology, University Hospital Münster, Germany
    • ,
  • Bodo Levkau, MD

      Affiliations

    • Division of Pathophysiology, Department of Internal Medicine, University Hospital Essen, University of Duisburg-Essen, Germany
    • ,
  • Jörg Stypmann, MD

      Affiliations

    • Department of Cardiology and Angiology, University Hospital Münster, Germany
    • ,
  • Christof Schmid, MD

      Affiliations

    • Department of Thoracic and Cardiovascular Surgery, University Hospital Münster, Germany.
    • ,
  • Kurt Werner Schmid, MD

      Affiliations

    • Department of Pathology and Neuropathology, University Hospital Essen, University of Duisburg-Essen, Germany
    • ,
  • Hideo Andreas Baba, MD

      Affiliations

    • Department of Pathology and Neuropathology, University Hospital Essen, University of Duisburg-Essen, Germany
    • Corresponding Author InformationReprint requests: Hideo Andreas Baba, MD, Institute of Pathology, University Hospital of Essen, University of Duisburg-Essen, Hufelandstr. 55, 45122 Essen, Germany

Received 10 May 2006 ,Revised 29 June 2006 ,Accepted 31 July 2006.

  • Image Result

    Significant decrease of cardiomyocyte diameter by LVAD as calculated by nonparametric Wilcoxon test (left). Significant difference between post-LVAD specimens compared with controls as calculated by p

    Significant decrease of cardiomyocyte diameter by LVAD as calculated by nonparametric Wilcoxon test (left). Significant difference between post-LVAD specimens compared with controls as calculated by post hoc analysis according to Duncan (right). Data (box plots). Outliers (circles). Significant differences (horizontal bars). Note that cardiomyocyte diameters after LVAD insertion remain higher than in control hearts (A). Significant decrease of COX-2–positive cardiomyocytes after LVAD insertion (left). No significant difference between COX-2–negative cardiomyocytes before and after LVAD insertion (right) (Wilcoxon test) (B). Significant decrease of cardiomyocytes with expression of COX-2 (C) and p-Akt (Thr308) (D) after LVAD insertion compared with controls. Data (box plots). Outliers (circles). Significant differences (horizontal bars).

  • Image Result
    Significant decrease of cardiomyocytes with expression of p-Akt (Ser473) (A) and p-Erk 1/2 (B) after LVAD insertion. Data (box plots). Outliers (circles). Significant differences (horizontal bars). No

    Significant decrease of cardiomyocytes with expression of p-Akt (Ser473) (A) and p-Erk 1/2 (B) after LVAD insertion. Data (box plots). Outliers (circles). Significant differences (horizontal bars). Note that p-Erk 1/2, although significantly reduced, does not decrease to the level of controls (post hoc analysis according to Duncan). Significant positive correlation between COX-2 and p-Akt (Thr308) protein expression (C) and COX-2 expression and cardiomyocyte diameter before LVAD insertion (D).

  • Image Result
    Immunofluorescence doublestaining. Failing myocardium before LVAD insertion: nuclei highlighted in blue (DAPI) (A). p-Akt (Thr308) highlighted in green (fluorescein isothiocyanate) predominantly in th

    Immunofluorescence doublestaining. Failing myocardium before LVAD insertion: nuclei highlighted in blue (DAPI) (A). p-Akt (Thr308) highlighted in green (fluorescein isothiocyanate) predominantly in the cardiomyocyte nuclei (green arrows) (B). COX-2 highlighted in red by Cy-3 with granular cytoplasmic distribution (red arrows) (C). Merged images demonstrating colocalization and cytoplasmic overlay of COX-2 and p-Akt (Thr308) in a subset of cardiomyocytes (yellow arrows) (D).

 This study was supported by the Deutsche Forschungsgemeinschaft to H. A. Baba (Ba1730/9-1), C. Schmid, and C. Vahlhaus (Va156/5-2).

PII: S0022-5223(06)01701-6

doi: 10.1016/j.jtcvs.2006.07.042

The Journal of Thoracic and Cardiovascular Surgery
Volume 133, Issue 1 , Pages 37-43 , January 2007

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