Data acquisition for the histoculture drug response assay in lung cancer

Yoshimasu, Tatsuya; Oura, Shoji; Hirai, Issei; Tamaki, Takeshi; Kokawa, Yozo; Hata, Kazuhito; Ohta, Fuminori; Nakamura, Rie; Kawago, Mitsumasa; Tanino, Hirokazu; Okamura, Yoshitaka; Furukawa, Tomoko

doi:10.1016/j.jtcvs.2006.06.030

« Previous Next »The Journal of Thoracic and Cardiovascular Surgery
Volume 133, Issue 2 , Pages 303-308, February 2007

Data acquisition for the histoculture drug response assay in lung cancer

Tatsuya Yoshimasu, MD, PhD
- Department of Thoracic and Cardiovascular Surgery, Wakayama Medical University, Wakayama, Japan
- Address for reprints: Tatsuya Yoshimasu, MD, PhD, Department of Thoracic and Cardiovascular Surgery, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-8509, Japan.
Shoji Oura, MD, PhD
- Department of Thoracic and Cardiovascular Surgery, Wakayama Medical University, Wakayama, Japan
Issei Hirai, MD, PhD
- Department of Thoracic and Cardiovascular Surgery, Wakayama Medical University, Wakayama, Japan
Takeshi Tamaki, MD
- Department of Thoracic and Cardiovascular Surgery, Wakayama Medical University, Wakayama, Japan
Yozo Kokawa, MD, PhD
- Department of Thoracic and Cardiovascular Surgery, Wakayama Medical University, Wakayama, Japan
Kazuhito Hata, MD
- Department of Thoracic and Cardiovascular Surgery, Wakayama Medical University, Wakayama, Japan
Fuminori Ohta, MD
- Department of Thoracic and Cardiovascular Surgery, Wakayama Medical University, Wakayama, Japan
Rie Nakamura, MD
- Department of Thoracic and Cardiovascular Surgery, Wakayama Medical University, Wakayama, Japan
Mitsumasa Kawago, MD
- Department of Thoracic and Cardiovascular Surgery, Wakayama Medical University, Wakayama, Japan
Hirokazu Tanino, MD, PhD
- Department of Thoracic and Cardiovascular Surgery, Wakayama Medical University, Wakayama, Japan
Yoshitaka Okamura, MD, PhD
- Department of Thoracic and Cardiovascular Surgery, Wakayama Medical University, Wakayama, Japan
Tomoko Furukawa, MD, PhD
- Analytical Center for Medical Science, SRL, Inc, Tokyo, Japan.

Received 18 December 2005; received in revised form 17 May 2006; accepted 15 June 2006.

Objective

Application of the histoculture drug response assay for lung cancer was investigated by using data acquired from lung cancer specimens.

Methods

From May 1994 through February 2005, histoculture drug response assay data were obtained from 359 lung cancer specimens held in our institute. We examined chemosensitivities of the tissues to cisplatin, doxorubicin, mitomycin C, 5-fluorouracil, docetaxel, paclitaxel, etoposide, irinotecan, and gemcitabine. Cutoff inhibition rates were determined with each drug for non–small cell lung cancer and were used to calculate predictabilities for chemotherapy responses.

Results

The evaluability of the histoculture drug response assay was high at 97.4%. Good predictability, including true-positive and true-negative rates of 73.2% and 100%, respectively, with an accuracy of 83.0%, was observed.