Surfactant abnormalities after single lung transplantation in dogs: impact of bronchoscopic surfactant administration

Relative amount (in percent of all phosphatidylcholine molecular species, left panel) and absolute concentration (right panel) of dipalmitoylated phosphatidylcholine (DPPC) molecular species from BALF of treated and untreated grafts and native lungs during reperfusion over 12 hours. Depicted are the mean ± SEM of 6 independent experiments. *P < .05, **P < .01, comparison between untreated and treated organs. The 0-hour values of the native lung represent the baseline values (excised lung from the recipient) and the 0-hour values of the graft represent the postischemia values (obtained by bronchoalveolar lavage [BAL] of the nontransplanted lung at the end of the ischemia period).

Minimum surface tension of large surfactant aggregates in absence (left panel) and in presence of proteins (right panel) from treated and untreated grafts and native lungs during reperfusion over 12 hours. Depicted are the mean ± SEM of 6 independent experiments. Reconstitution with bronchoalveolar lavage–derived proteins was performed as detailed in the Methods section. Surface activity was assessed in the Pulsating Bubble Surfactometer at 2 mg/mL phospholipid always. *P < .05, ***P < .001, comparison between untreated and treated organs; ⁺⁺P < .01), ⁺⁺⁺P < .001, comparison between baseline and the untreated donor lung. The 0-hour values of the native lung represent the baseline values (excised lung from the recipient) and the 0-hour values of the graft represent the postischemia values (obtained by BAL of the nontransplanted lung at the end of the ischemia period).

Correlation of all data sets of relative palmitic acid concentration in PC and minimum surface tension, as assessed in the non–surfactant-treated lungs.