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Volume 127, Issue 2 , Pages 329-334 , February 2004

Slow release of bone morphogenetic protein 2 from a gelatin sponge to promote regeneration of tracheal cartilage in a canine model

Taku Okamoto, MD
- Second Department of Surgery, Kagawa Medical University, Kagawa, Japan
Yasumichi Yamamoto, MD
- Second Department of Surgery, Kagawa Medical University, Kagawa, Japan
Masashi Gotoh, MD
- Second Department of Surgery, Kagawa Medical University, Kagawa, Japan
Cheng-Long Huang, MD
- Second Department of Surgery, Kagawa Medical University, Kagawa, Japan
Tatsuo Nakamura, MD
- Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
Yasuhiko Shimizu, MD
- Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
Yasuhiko Tabata, PhD
- Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
Hiroyasu Yokomise, MD
- Second Department of Surgery, Kagawa Medical University, Kagawa, Japan
- Address for correspondence: Hiroyasu Yokomise, MD, Second Department of Surgery, Kagawa Medical University, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan

Received 28 May 2003 ,Revised 11 August 2003 ,Accepted 19 August 2003.

Surgical procedure for control group: 1 × 5-cm gap in tracheal cartilage was created by removing 5-cm long strips of 10 sequential cartilage rings, taking care not to damage mucosa.

Surgical procedure for control group: 1 × 5-cm gap in tracheal cartilage was created by removing 5-cm long strips of 10 sequential cartilage rings, taking care not to damage mucosa.
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Surgical procedures for gelatin sponge and BMP groups: gap in tracheal cartilage was created as shown in Figure 1. Gelatin sponge soaked in buffer solution alone or solution containing 12 μg BMP-2 was

Surgical procedures for gelatin sponge and BMP groups: gap in tracheal cartilage was created as shown in Figure 1. Gelatin sponge soaked in buffer solution alone or solution containing 12 μg BMP-2 was then implanted into gap.
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Bronchoscopic findings in control group 6 months after surgery. Tracheal lumen collapsed during coughing. a, At rest; b, during coughing.

Bronchoscopic findings in control group 6 months after surgery. Tracheal lumen collapsed during coughing. a, At rest; b, during coughing.
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Bronchoscopic findings in gelatin sponge group 6 months after surgery. Tracheal lumen collapsed during coughing. a, At rest; b, during coughing.

Bronchoscopic findings in gelatin sponge group 6 months after surgery. Tracheal lumen collapsed during coughing. a, At rest; b, during coughing.
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Bronchoscopic findings in BMP group 6 months after surgery. Slight tracheal stenosis was observed, but there was no collapse, even during coughing. a, At rest; b, during coughing.

Bronchoscopic findings in BMP group 6 months after surgery. Slight tracheal stenosis was observed, but there was no collapse, even during coughing. a, At rest; b, during coughing.
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Microscopic findings in control group 6 months after surgery. No cartilage regeneration is detectable around cartilageal stump (arrow). (Original magnification ×150).

Microscopic findings in control group 6 months after surgery. No cartilage regeneration is detectable around cartilageal stump (arrow). (Original magnification ×150).
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Microscopic finding in gelatin sponge group 6 months after implantation. No cartilage regeneration is detectable around cartilageal stump (arrow). (Original magnification ×150).

Microscopic finding in gelatin sponge group 6 months after implantation. No cartilage regeneration is detectable around cartilageal stump (arrow). (Original magnification ×150).
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Microscopic finding for BMP group 6 months after implantation. Regenerated cartilage (thin arrow) arising from perichondrium can be seen around stump (thick arrow) of resected cartilagines. (Original

Microscopic finding for BMP group 6 months after implantation. Regenerated cartilage (thin arrow) arising from perichondrium can be seen around stump (thick arrow) of resected cartilagines. (Original magnification ×150).
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